MersV1, Main, Exploration, bibRecord, 003526

PNA oligomers as tools for specific modulation of gene expression

Identifieur interne : 003526 ( Main/Exploration ); précédent : 003525; suivant : 003527

PNA oligomers as tools for specific modulation of gene expression

Auteurs : Margus Pooga [Suède, Estonie] ; Tiit Land [Suède] ; Tamas Bartfai [États-Unis] ; Ülo Langel [Suède, États-Unis]

Source :

Biomolecular Engineering [ 1389-0344 ] ; 2001.

RBID : ISTEX:6D7C4F13EC527346B05A24550DC1FAAA9668F8C8

English descriptors

KwdEn :
- Antigene, Antisense, PNA, PNA delivery.
Teeft :
- Acad, Antigene, Antisense, Antisense agent, Antisense application, Antisense effect, Antisense effects, Antisense pnas, Antisense properties, Binding sites, Biochem pharmacol, Biochim biophys acta, Bioconjug chem, Biol chem, Biomolecular, Biomolecular engineering, Bowes melanoma cells, Brain barrier, Cell surface, Cell viability, Cellular, Cellular delivery, Cellular uptake, Chem, Coding region, Duplex, Elsevier science, Enzymatic activity, Eukaryotic systems, Febs lett, Galanin binding sites, Galr1 mrna, Gene expression, High concentration, High concentrations, Higher stability, Inducible nitric oxide synthase, Intact cells, Intrathecal administration, Lett, Limited amount, Long incubation times, Mammalian cells, Mrna, Mrna concentration, Nanomolar concentrations, Natl, Neuronal cells, Neurotensin, Nielsen, Nuclear delivery, Nuclear microinjection, Nuclear uptake, Nucleic, Nucleic acids, Oligomer, Oligomers, Oligomers bind, Oligonucleotides, Other hand, Pantp, Peptide, Plasma membrane, Plasmid, Pnas, Pooga, Proc, Proc natl acad, Prostatic carcinoma cells, Protein concentration, Rabbit reticulocyte lysate, Receptor, Receptor agonist, Receptor mrna, Receptor protein, Respective enzymes, Rnase, Spinal cord, Strand invasion, Strong antisense effect, Strong decrease, Telomerase, Telomerase activity, Telomere length, Template region, Transcription, Translational level, Uorescent protein, Uptake.

Abstract

Abstract: Small synthetic molecules that can specifically inhibit translation and/or transcription have shown great promise as potential antisense/antigene drugs. Peptide nucleic acid (PNA), an oligonucleotide mimic, has a non-charged achiral polyamide backbone to which the nucleobases are attached. PNA oligomers are extremely stable in biological fluids and they specifically hybridise to DNA or RNA in a complementary manner, forming very strong heteroduplexes. Some of the mRNAs have yet undetermined and possibly long half-lives, successful down regulation of gene expression by antisense oligonucleotides (ON) requires that the antisense agent is long lived. PNA fulfils this requirement better than phosphodiester or phsphorothioate ONs. PNA can inhibit transcription and translation of respective genes by tight binding to DNA or mRNA. First in vitro experiments to specifically down regulate protein expression by PNA have been followed by successful antisense and antigene application of PNA oligomers in vivo. This review discusses the principles of the in vitro and in vivo use of PNA oligonucleotides.

Url:

https://api.istex.fr/ark:/67375/6H6-9MHJFRJ0-B/fulltext.pdf

DOI: 10.1016/S1389-0344(01)00075-2

Affiliations:

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Le document en format XML

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<term>Antigene</term>
<term>Antisense</term>
<term>Antisense agent</term>
<term>Antisense application</term>
<term>Antisense effect</term>
<term>Antisense effects</term>
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<term>Antisense properties</term>
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<term>Bioconjug chem</term>
<term>Biol chem</term>
<term>Biomolecular</term>
<term>Biomolecular engineering</term>
<term>Bowes melanoma cells</term>
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<term>Cell surface</term>
<term>Cell viability</term>
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<term>Cellular uptake</term>
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<term>Proc natl acad</term>
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<front><div type="abstract" xml:lang="en">Abstract: Small synthetic molecules that can specifically inhibit translation and/or transcription have shown great promise as potential antisense/antigene drugs. Peptide nucleic acid (PNA), an oligonucleotide mimic, has a non-charged achiral polyamide backbone to which the nucleobases are attached. PNA oligomers are extremely stable in biological fluids and they specifically hybridise to DNA or RNA in a complementary manner, forming very strong heteroduplexes. Some of the mRNAs have yet undetermined and possibly long half-lives, successful down regulation of gene expression by antisense oligonucleotides (ON) requires that the antisense agent is long lived. PNA fulfils this requirement better than phosphodiester or phsphorothioate ONs. PNA can inhibit transcription and translation of respective genes by tight binding to DNA or mRNA. First in vitro experiments to specifically down regulate protein expression by PNA have been followed by successful antisense and antigene application of PNA oligomers in vivo. This review discusses the principles of the in vitro and in vivo use of PNA oligonucleotides.</div>
</front>
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PNA oligomers as tools for specific modulation of gene expression

PNA oligomers as tools for specific modulation of gene expression

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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